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Test Code BART Bartonella Antibody Panel, IgG and IgM, Serum

Reporting Name

Bartonella Ab Panel, IgG and IgM

Useful For

Diagnosis of Bartonella infection, especially in the context of a cat scratch

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Specimen Minimum Volume

0.15 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days

Reference Values

BARTONELLA HENSELAE

IgG: <1:128

IgM: <1:20

 

BARTONELLA QUINTANA

IgG: <1:128

IgM: <1:20

Day(s) Performed

Monday through Saturday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86611 x 4

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BART Bartonella Ab Panel, IgG and IgM 90251-0

 

Result ID Test Result Name Result LOINC Value
15659 Bart Henselae IgG In Process
15660 Bart Henselae IgM In Process
15661 Bart Quintana IgG In Process
15662 Bart Quintana IgM In Process

Method Description

The Euroimmun indirect immunofluorescence test is a standardized, in vitro assay for the determination of specific antibodies against Bartonella henselae and Bartonella quintana. BIOCHIP Mosaics are coated with B henselae and B quintana infected cells positioned next to each other in one reaction field. Samples are diluted and incubated on the substrate slides. If the reaction is positive, specific antibodies of class IgG and IgM attach to the antigens. In a second step, the attached antibodies are stained with fluorescein-labeled anti-human antibodies and made visible using fluorescence microscopy. Semiquantitative endpoint titers are obtained by testing serial dilutions of positive specimens.(Unpublished Mayo method)

Report Available

Same day/1 to 3 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Method Name

Immunofluorescence Assay (IFA)

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.