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Test Code BRCMG Brucella Antibody Screen, IgM and IgG, ELISA, Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Secondary ID

607346

Useful For

Evaluating patients with suspected brucellosis

Profile Information

Test ID Reporting Name Available Separately Always Performed
BRCM Brucella Ab Screen, IgM ELISA, S No Yes
BRCG Brucella Ab Screen, IgG ELISA, S No Yes
BRCI Brucella Ab Screen Interpretation No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
BRUTA Brucella Ab, Agglutination, S Yes No

Testing Algorithm

If the Brucella antibody screen, IgM or IgG, result is either positive or equivocal, then confirmation by Brucella total antibody agglutination testing will be performed at an additional charge.

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Brucella Ab Screen, IgM/IgG ELISA, S

Specimen Type

Serum

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Heat-inactivated specimen Reject

Reference Values

IgG SCREEN

Negative

 

IgM SCREEN

Negative

Reference values apply to all ages.

Method Description

Serum is tested using an enzyme-linked immunosorbent assay (ELISA) Test Kit containing microtiter strips with wells coated with Brucella abortus antigens (strain W99). In the first reaction step, diluted patient samples are incubated in the wells. Specific IgG or IgM antibodies, if present in the serum, will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labeled antihuman IgG or antihuman IgM (enzyme conjugate). After addition of the substrate, tetramethylbenzidine/hydrogen peroxide and a sulphuric acid stop solution, the resulting color reaction is measured photometrically at a wavelength of 450nm.(Package insert: Anti-Brucella abortus ELISA Test Instructions, Euroimmun Medizinische Labordiagnostika; IgM: 01//2019, IgG: 08/2020)

Day(s) Performed

Tuesday, Thursday

Report Available

Same day/1 to 5

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86622 x 2-Brucella antibody, IgG and IgM

86622-Brucella total antibody, agglutination (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BRCMG Brucella Ab Screen, IgM/IgG ELISA, S 91140-4

 

Result ID Test Result Name Result LOINC Value
BRCM Brucella Ab Screen, IgM ELISA, S 24388-1
BRCG Brucella Ab Screen, IgG ELISA, S 24387-3
BRCI Brucella Ab Screen Interpretation 66485-4

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.