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HelpTest Code CD3+ CD3+
Aliases (synonyms)
Absolute CD3 Enumeration
Useful For
Quantification of CD3+ cells immediately prior to CAR-T sequestration.
Patient Preparation
No special patient preparation required
Collection Method
Venous draw
Minimum Volume
2 mL
Container
Purple (EDTA)
Preferred Specimen
Whole Blood
Acceptable Specimens
EDTA Whole Blood
Reference or Target Ranges
Adult Reference Values:
| Components | Units | Reference Range |
| % CD3 T Cells | % | 58.0 - 86.0 |
| CD3 T Cells | cells/μL | 550.0 - 2271.0 |
Pediatric Reference Values:
| Components | 5-8 mo. | 9-14 mo. | 15-23 mo. | 2-4 yrs. | 5-9 yrs. | 10-15 yrs. |
| % CD3 T Cells | 49-95 | 56-87 | 36-92 | 52-92 | 55-97 | 52-90 |
| CD3 T Cells (cells/μL) | 1400-11500 | 2400-8300 | 700-8800 | 850-4300 | 770-4000 | 850-3200 |
Reportable Units
cells/μL
Critical Value
Not defined for this assay
Reasons for Rejection
| Hemolysis Threshold | N/A |
| Icterus Threshold | N/A |
| Lipemia Threshold | N/A |
| Other | QNS, contaminated, improperly labled, improper specimen type, specimen clotted, specimen refrigerated |
Specimen Stability
| Ambient | 24 hours |
| Refrigerated (4°C to 8°C) | Do not refrigerate |
| Frozen(-40°C to 0°C) | Do not freeze |
Performance Information
| Days and Time Performed | Monday – Friday 9AM – 5PM |
| Expected Turn Around Time | 2 hours |
| Stat Availabilty | No |
| Performing Bench | UH BD Lyric |
| Methodology/Method Description | Flow Cytometric evaluation, Panel Components (CD3, CD45, 7 AAD) |
CPT Codes
86359