Method Description

The ZEUS ELISA EBV (Epstein Barr virus) VCA (viral capture antigen) IgM, VCA IgG, and EBNA-1 (Epstein Barr nuclear antigen-1) IgG Test Systems are designed to detect IgM, IgG, IgG class antibodies to EBNA in human sera. Creation of the sensitized wells of the plastic microwell strips occurred using passive adsorption with EBV antigen. The test procedure involves three incubation steps. Test sera are diluted with the Sample Diluent provided. The IgM Sample Diluent contains antihuman IgG that precipitates and removes IgG and rheumatoid factor from the sample leaving IgM free to react with the immobilized antigen. During sample incubation any antigen specific antibody in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components. Peroxidase Conjugated goat ant-human IgM or IgG is added to the wells and the plate is incubated. The Conjugate will react with IgM or IgG antibody immobilized on the solid phase. The wells are washed to remove unbound Conjugate. The microwells containing immobilized peroxidase Conjugate are incubated with peroxidase Substrate Solution. Hydrolysis of the Substrate by peroxidase produces a color change. After a period of time the reaction is stopped and the color intensity of the solution is measured photometrically. The color intensity of the solution depends upon the antibody concentration in the original test sample.(Package inserts: EBV-VCA IgM Test System. Zeus Scientific, Inc.; 12/13/2017; EBV-VCA IgG Test System. Zeus Scientific, Inc.; 12/19/2017; EBNA-1 IgG Test System. Zeus Scientific, Inc.; 12/19/2017)