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HelpTest Code GAST Gastrin, Serum
Reporting Name
Gastrin, SUseful For
Investigation of patients with achlorhydria or pernicious anemia
Investigation of patients suspected of having Zollinger-Ellison syndrome
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Diagnosis of gastrinoma
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Patient Preparation:
1. Fasting (8 hours) required
2. For 12 hours before specimen collection, patient should not take multivitamins or dietary supplements (eg, hair, skin, and nail supplements) containing biotin (vitamin B7).
3. For 1 week before specimen collection, if medically feasible, patient should not take proton pump inhibitors (omeprazole, lansoprazole, dexlansoprazole, esomeprazole, pantoprazole, and rabeprazole).
4. For at least 2 weeks before specimen collection, patient should not take or receive drugs that interfere with gastrointestinal motility (eg, opioids).
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. If multiple specimens are collected, submit each vial under a separate order.
2. Label specimens with corresponding collection time.
3. Centrifuge at within 2 hours of collection. Refrigerated centrifugation is preferred but not required. Immediately aliquot serum into plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 30 days | |
| Refrigerated | 24 hours |
Reference Values
<100 pg/mL
There is no evidence that fasting serum gastrin levels differ between adults and children. Although 8-hour fasts are difficult or impossible to enforce in small children, serum gastrin levels after shorter fasting periods (3-8 hours) may be 50% to 60% higher than the 8-hour fasting value.
For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html.
Day(s) Performed
Monday through Saturday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
82941
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| GAST | Gastrin, S | 2333-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| GAST | Gastrin, S | 2333-3 |
Method Description
The Immulite 2000 Gastrin assay is a chemiluminescent, enzyme-labeled immunometric assay based on a ligand-labeled murine monoclonal capture antibody specific for gastrin and separation by antiligand-coated solid phase. The patient sample along with the ligand-labeled, anti-gastrin monoclonal antibody, an alkaline phosphatase-conjugated rabbit polyclonal anti-gastrin antibody, and an alkaline phosphatase-conjugated murine monoclonal anti-gastrin antibody are simultaneously incubated in the presence of the immobilized antiligand bead in a reaction tube. During the 60-minute incubation, gastrin molecules in the sample form antibody sandwich complexes that, in turn, bind to antiligand on the solid phase. Unbound conjugate is then removed by a centrifugal wash, after which luminogenic substrate is added, and the reaction tube is incubated for an additional 5 minutes. The chemiluminescent substrate, a phosphate ester of adamantyl dioxetane, undergoes hydrolysis in the presence of alkaline phosphatase to yield an unstable intermediate. The continuous production of the intermediate results in the sustained emission of light. The bound complex and, thus, also the photon output, as measured by the luminometer is proportional to the concentration of gastrin in the sample.(Instruction manual: Immulite 2000 Gastrin. Siemens Medical Solutions Diagnostics; PIL2KGA-15, 08/2020)
Report Available
1 to 3 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Automated Chemiluminescent Immunometric Assay
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Oncology Test Request Form (T729)