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Test Code HbsaQnt Hepatitis B Surface Antibody, Quant/Qual 

Useful For

The Atellica® IM Anti-Hepatitis B surface Antigen 2 (aHBs2) assay is an in vitro diagnostic immunoassay for the qualitative and quantitative determination of total antibodies to hepatitis B surface antigen in human adult, adolescent, and pediatric serum or plasma (EDTA, lithium heparinized, or sodium heparinized) and neonatal samples using the Atellica® IM Analyzer. The assay results may be used as an aid in the determination of susceptibility to hepatitis B virus (HBV) infection in individuals prior to or following HBV vaccination or where vaccination status is unknown. Assay results may be used with other HBV serological markers for the laboratory diagnosis of HBV disease associated with HBV infection. A reactive assay result will allow a differential diagnosis in individuals displaying signs and symptoms of hepatitis in whom etiology is unknown. 

Patient Preparation

No special patient preparation required

Collection Method

Venous collection

Minimum Volume

1 mL

Preferred Specimen

Serum

Acceptable Specimens

Serum:

  • Gold Top SST
  • Red Top

Plasma:

  • Purple or Pink Top(K2/K3 EDTA)
  • Light Green (Lithium heparin) 
  • Dark Green (Sodium heparin)

Reference or Target Ranges

Nonreactive

Reportable Units

mIU/mL

Critical Value

Not defined for this assay

Reasons for Rejection

 

Hemolysis Threshold 500 mg/dL 
Icterus Threshold 40 mg/dL
Lipemia Threshold 1000 mg/dL
Other QNS, contaminated, improperly Labeled, improper specimen type

Specimen Stability

Ambient 24 hours
Refrigerated (4°C to 8°C) Serum & Plasma: 4 days
Frozen(-40°C to 0°C) 3 months

Performance Information

Days and Time Performed 24/7
Expected Turn Around Time Routine: 4 hours
Stat Availability No
Performing Bench UH Chemistry
Methodology/Method Description Siemens Atellica – Direct Chemiluminescent Immunoassay 

Special Handling

Samples that have been frozen or contain precipitates should be centrifuged prior to performing the assay.

Additional Information/Important Notes

Result Clarification

 

Nonreactive: Samples with an initial value <8.0 mIU/mL.  Anti-HBS is <10.0 mIU/mL and the patient is considered not to have protective immunity to Hepatitis B Virus. 


Reactive: Samples with an initial value ≥ 12.0 mIU/mL.  Anti-HBs is detected at ≥ 10.0 mIU/mL and the patient is considered to have protective immunity to Hepatitis B Virus infection. 

CPT Codes

86317