Home
HelpTest Code Hgb S Hemoglobin S Electrophoresis
Aliases
Hemoglobin S Quantitation without Pathologist Interpretation
Useful For
Analysis of sickle cell disease, trait, treatments, and thalassemias.
Patient Preparation
No special patient preparation required
Collection Method
Venous collection
Minimum Volume
1 mL
Container
Purple / EDTA
Preferred Specimen
EDTA Whole Blood
Acceptable Specimens
EDTA Whole Blood
Reference or Target Ranges
| Hemoglobin A | 96 – 98.5 |
| Hemoglobin F | 0 |
| Hemoglobin S | 0 |
| Hemoglobin A2 | 1.5 – 4.0 |
| Hemoglobin C | 0 |
Reportable Units
% (percentage)
Critical Value
Not defined for this assay
Reasons for Rejection
| Hemolysis Threshold |
N/A |
| Icterus Threshold |
N/A |
| Lipemia Threshold |
N/A |
| Other |
QNS, contaminated, improperly labled, improper specimen type. Avoid aged, improperly stored blood samples; degradation products (or artefacts) may affect the electrophoretic pattern after 7 days storage. |
Specimen Stability
| Ambient |
N/A |
| Refrigerated (2°C to 8°C) |
7 Days |
| Frozen(<-20°C to 0°C) |
N/A |
Performance Information
| Days and Time Performed |
Monday – Friday 8 AM – 4:30 PM |
| Expected Turn Around Time |
Within 3 days of arrival in lab |
| Stat Availabilty |
No |
| Performing Bench |
Special Chemistry |
| Methodology/Method Description |
Sebia Capillarys 2 – Capillary electrophoresis |
Additional Information/Important Notes
- This method has not been validated in the neonate/newborn population, birth to 28 days of age. Repeat hemoglobin electrophoresis at 4 month of age may be suggested.
- When analyzing blood samples from transfused patients with sickle cell disease, with low Hb A level (<10%), Hb S fraction may appear shifted from Z(S) zone to Z(D) zone. It is necessary to analyze the hematologic state and to perform complementary studies in order to confirm the presence of Hb S.