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HelpTest Code Insul Insulin Level Total
Aliases
Total Insulin
Useful For
IVD test use for the quantitative measurement of Insulin in human serum. Circulating insulin concentration in serum or plasma provides important information for the estimation of insulin secretion and insulin resistance.
Patient Preparation
Fasting at the request of physician
Special Collection Instructions
Allow specimen to clot completely at room temperature for 45 min, then centrifuge. Separate serum from cells ASAP then freeze serum.
Collection Method
Venous collection
Minimum Volume
0.5 mL serum
Container
Red / Additive Free
Preferred Specimen
Frozen Serum
Acceptable Specimens
Frozen Serum
Reference or Target Ranges
| Fasting | 1 – 14 μIU/mL |
| 120 min | 1 – 35 μIU/mL |
Reportable Units
μIU/mL
Critical Value
Not defined for this assay
Reasons for Rejection
| Hemolysis Threshold |
Reject hemolyzed samples. Hemolysis may falsely lower the value due to insulin degrading enzyme in red blood cells |
| Icterus Threshold |
N/A |
| Lipemia Threshold |
No Interference up 1,600 mg/dL |
| Interfering Subtances/Other |
Wrong container, labeled improperly, serum not frozen |
Specimen Stability
| Ambient |
N/A |
| Refrigerated (2°C to 8°C) |
1 day |
| Frozen(-70°C to 0°C) |
60 days |
Performance Information
| Days and Time Performed |
Monday – Friday 8 AM – 5 PM |
| Expected Turn Around Time |
5 days excluding weekends and holidays |
| Stat Availabilty |
Per request of clinician |
| Performing Bench |
Diagnostic Diabetes Lab |
| Methodology/Method Description |
Tosoh AIA-900 – Two-site immunoenzymometric assay |
Special Handling
Allow specimen to clot completely at room temperature for 45 min, then centrifuge. Separate serum from cells ASAP and freeze serum. Avoid repeated freeze/thaw cycles.
Additional Information/Important Notes
For patients that are being treated with Asfotase Alfa (Strensiq®) elevated test results are likely to occur using this method. Results should not be used for patients receiving Asfotase Alfa treatment. An alternative test method should be used that does not utilize alkaline phosphatase technology. All results should be interpreted with respect to the clinical picture of the patient
CPT Codes
83525