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Test Code LID Lidocaine, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.


Secondary ID

8382

Useful For

Assessing optimal lidocaine dosing during the acute management of ventricular arrhythmias following myocardial infarction or during cardiac manipulation such as surgery

 

Assessing potential lidocaine toxicity

Method Name

Enzyme-Multiplied Immunoassay Technique (EMIT)

Reporting Name

Lidocaine, S

Specimen Type

Serum Red

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Refrigerated (preferred) 14 days
  Ambient  14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject

Reference Values

Therapeutic: 1.5-5.0 mcg/mL

Critical value: >6.0 mcg/mL

Method Description

The enzyme-multiplied immunoassay technique assay is a homogeneous enzyme immunoassay technique used for the analysis of specific compounds in biological fluids. The assay is based on competition between drug in the sample and drug labeled with the enzyme glucose-6-phosphate dehydrogenase (G6PD) for antibody binding sites. Enzyme activity decreases upon binding to the antibody, so the drug concentration in the sample can be measured in terms of enzyme activity. Active enzyme converts oxidized nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change, which is measured spectrophotometrically. Endogenous serum G6PD does not interfere because the coenzyme functions only with the bacterial (Leuconostoc mesenteroides) enzyme employed in the assay.(Package insert: Lidocaine reagent. Seimens Healthcare Diagnostics, Ltd; 04/2015)

Day(s) Performed

Monday through Saturday

Report Available

Same day/1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

80176

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LID Lidocaine, S 3714-3

 

Result ID Test Result Name Result LOINC Value
8382 Lidocaine, S 3714-3

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.