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Test Code Lamel Lamellar Body Count

Aliases

Amniotic Fluid Lamellar Body

Useful For

Lamellar bodies are packets of pulmonary surfactant present in amniotic fluid. They are the secretory granules of Type II pneumatocytes, and their presence in amniotic fluid is a useful predictor of fetal lung maturity. The higher the lamellar body count the greater the likelihood of fetal lung maturity.

Special Collection Instructions

The amniotic fluid must be free of mucous, meconium or blood

Collection Method

Sterile- protect from light

Minimum Volume

1 mL

Preferred Specimen

Amniotic fluid 10 mL

Acceptable Specimens

Amniotic fluid

Reference or Target Ranges

Lamellar Body Count Interpretation
0 – 15 × 103 particles/uL Immature (high risk of RDS*)
16 – 49 × 103 particles/uL Intermediate
>50 × 103 particles/uL Mature (low risk of RDS)

Reportable Units

particles /uL

Reasons for Rejection

Hemolysis Threshold N/A
Icterus Threshold N/A
Lipemia Threshold N/A
Other Clotted; QNS, Contaminated; Improperly labeled

Specimen Stability

Ambient 7 days
Refrigerated (4°C to 8°C) 28 days
Frozen(-70°C to 0°C) N/A

Performance Information

Days and Time Performed 24/7
Expected Turn Around Time Stat within 1 hour of arrival in lab
Routine within 8 hours of arrival in lab
Stat Availabilty Yes
Performing Bench WCH Hematology
Methodology/Method Description Sysmex

Special Handling

Room Temp

Additional Information/Important Notes

The amniotic fluid should not be visibly contaminated by blood, mucous or meconium, and should be obtained by amniocentesis if possible, and not from vaginal pooling. Amniotic fluid from vaginal pooling should only be used for this test if the sample is obtained immediately after membrane rupture, and is immediately put on ice. As little as 1% blood contamination can decrease the lamellar body count by as much as 20%, and mucous and meconium can cause highly erroneous results. Specimens containing mucous, meconium, or blood must be rejected.

CPT Codes

83664