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Test Code PMET Metanephrines, Fractionated, Free, Plasma

Reporting Name

Metanephrines, Fract., Free, P

Useful For

Screening test for presumptive diagnosis of catecholamine-secreting pheochromocytomas or paragangliomas

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Plasma EDTA


Specimen Required


Patient Preparation: Use of an Epi-pen within the last 7 days may produce inaccurate results.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Within 2 hours of collection centrifuge and aliquot plasma into a plastic vial.


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen (preferred) 28 days
  Refrigerated  14 days
  Ambient  7 days

Reference Values

METANEPHRINE, FREE

<0.50 nmol/L

 

NORMETANEPHRINE, FREE

<0.90 nmol/L

Day(s) Performed

Monday through Saturday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83835

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PMET Metanephrines, Fract., Free, P 57772-6

 

Result ID Test Result Name Result LOINC Value
10140 Normetanephrine, Free 40851-8
10139 Metanephrine, Free 49700-8

Method Description

Free metanephrine (MN) and normetanephrine (NMN) are extracted from plasma using solid phase extraction. The concentrated eluate is analyzed using liquid chromatography tandem mass spectrometry and quantified using stable isotope labeled internal standards, d3-MN and d3-NMN . Analytes and internal standards are ionized using electro spray ionization and are detected in the multiple reaction-monitoring mode. The specific transitions for MN, NMN, d3-MN, and d3-NMN are m/z 180.2 to m/z 148.2, 166.2 to m/z 134.2, 183.2 to m/z 151.2, and m/z 169.2 to m/z 137.2, respectively.(Unpublished Mayo method)

Report Available

2 to 5 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Oncology Test Request (T729)