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Test Code TRYPT Tryptase, Serum

Reporting Name

Tryptase, S

Useful For

Evaluation of individuals with suspected mast cell activation, which may occur as a result of anaphylaxis or allergen challenge

 

Evaluation of patients with suspected cutaneous or systemic mastocytosis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial

Additional Information: Tryptase degenerates very quickly when left in the presence of red blood cells.


Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 14 days
  Refrigerated  7 days

Reference Values

<11.5 ng/mL

Day(s) Performed

Monday through Friday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83520

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TRYPT Tryptase, S 21582-2

 

Result ID Test Result Name Result LOINC Value
TRYPT Tryptase, S 21582-2

Method Description

Anti-tryptase, covalently coupled to ImmunoCAP, reacts with tryptase in the patient serum specimen. After washing, enzyme-labeled antibodies against tryptase are added to form a complex. After incubation, unbound enzyme-labeled antibodies are washed away, and the bound complex is incubated with a developing agent. After stopping the reaction, the fluorescence in the eluate is measured. The fluorescence is directly proportional to the concentration of tryptase in the serum specimen.(Package insert: ImmunoCAP Tryptase. Phadia AB; 10/2019)

Report Available

2 to 5 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Method Name

Fluorescence Enzyme Immunoassay (FEIA)

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.

Secondary ID

81608

Testing Algorithm

For more information, see Mast Cell Disorder: Diagnostic Algorithm, Bone Marrow