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Test Code VWFMP von Willebrand Factor Multimer Analysis, Plasma


Specimen Required


Only orderable as part of a coagulation reflex. For more information see:

ALUPP / Lupus Anticoagulant Profile, Plasma

ALBLD / Bleeding Diathesis Profile, Limited, Plasma

AVWPR / von Willebrand Disease Profile, Plasma

 

For more information see Coagulation Guidelines for Specimen Handling and Processing.


Secondary ID

604411

Useful For

As a reflex component of several coagulation consultation unit codes, when indicated

 

When results of complementary laboratory tests are abnormally low or discordant (eg, F8A / Coagulation Factor VIII Activity Assay, Plasma; VWACT / von Willebrand Factor Activity, Plasma; and VWAG / von Willebrand Factor Antigen, Plasma)

 

To subtype von Willebrand disease (VWD) (primarily identify variants of type 2 VWD)

 

As an aid in determining appropriate treatment

Method Name

Only orderable as part of a coagulation reflex. For more information see:

ALUPP / Lupus Anticoagulant Profile, Plasma

ALBLD / Bleeding Diathesis Profile, Limited, Plasma

ACBL / Bleeding Diathesis Profile, Comprehensive, Plasma

AVWPR / von Willebrand Disease Profile, Plasma

 

Agarose Gel Electrophoresis

Reporting Name

von Willebrand Factor Multimer, P

Specimen Type

Plasma Na Cit

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 42 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

Only orderable as part of a coagulation reflex. For more information see:

ALUPP / Lupus Anticoagulant Profile, Plasma

ALBLD / Bleeding Diathesis Profile, Limited, Plasma

AVWPR / von Willebrand Disease Profile, Plasma

 

An interpretive report will be provided.

Method Description

Platelet-poor plasma proteins are denatured using heat and an anionic detergent, sodium dodecyl sulfate. The sample is then electrophoresed through a discontinuous agarose gel on a cooled horizontal electrophoresis unit overnight to separate the von Willebrand factor (VWF) multimers by size. The gel is fixed in acid and isopropanol, washed in water, and incubated with dilute rabbit antihuman VWF. After washing away unbound antibody, the gel is incubated with dilute goat antirabbit IgG antibody tagged with an infrared dye. Excess secondary antibody is washed away, and the gel is scanned using an infrared imaging system. The digitized image of the electrophoretic distribution of the VWF multimers is interpreted by a coagulation consultant and a written report is provided.(Ruggeri ZM, Zimmerman TS: Variant von Willebrand's disease. Characterization of two subtypes by analysis of multimeric composition of FVIII/vWF in plasma and platelets. J Clin Invest 1980;65:1318-1325; Favaloro EJ and Lippi G. eds. Hemostasis and Thrombosis, Methods and Protocols. Humana Press 2017)

Day(s) Performed

Monday, Tuesday

Report Available

7 to 14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85247

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VWFMP von Willebrand Factor Multimer, P 32217-2

 

Result ID Test Result Name Result LOINC Value
604411 von Willebrand Factor Multimer, P 32217-2
604412 VWF Multimer Interpretation 48595-3