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Test Code ANA Prof ANA Profile

Useful For

Aids in the diagnosis of systemic autoimmune diseases

Patient Preparation

No special patient preparation required

Collection Method

Venous collection

Minimum Volume

3.5 mL

Container

Gold Top Serum Separator

Preferred Specimen

Serum

Acceptable Specimens

Serum:

  • Gold Top SST

  • Red Top

Plasma:
  • Light Geen Top (lithium heparin)

Reference or Target Ranges

dsDNA Negative
RNP <20 RLU
Sm <20 RLU
SSARo52 <20 RLU
SSARo60 <20 RLU
SSB <20 RLU
Scl70 <20 RLU
Jo-1 <20 RLU

Critical Value

Not defined for this assay

Reasons for Rejection

Hemolysis Threshold
 N/A
Icterus Threshold
 N/A
Lipemia Threshold
 N/A
Other
 QNS, contaminated, improperly labeled, improper specimen type

Specimen Stability

Ambient
 48 Hours
Refrigerated (4°C to 8°C)
 14 Days
Frozen(-70°C to 0°C)
 Indefinitely

Performance Information

Days and Time Performed
 Monday – Friday 7 AM – 4 PM
Expected Turn Around Time
 Within 7 days of arrival in lab
Stat Availability
 No
Performing Bench
 ANA
Methodology/Method Description
 Manual and Bioflash – Indirect Immunofluorescence and Chemilluminescence

CPT Codes

86255; 86256 (if appropriate)

Panel Components

  • dsDNA
  • RNP
  • Sm
  • SSARo52
  • SSARo60
  • SSB
  • Scl70
  • Jo-1

Reflex Testing

dsDNA reflex to titer if positive