Test Code Antigen Typing Antigen Typing
Useful For
Antigen typing is performed on donor units to prevent transfusion of antigen positive red blood cells to patients with clinically significant alloantibodies. Antigen typing may also be performed on patient red blood cells to assist in antibody identification, HDN evaluation, phenotyping of sickle cell patients, phenotyping of patients with warm autoantibodies who have not been transfused within the previous three months, or as part of an organ transplant work-up.
Patient Preparation
No special patient preparation required
Special Collection Instructions
Sample labeling requirements:
For all patients over the age of four months, when sample is intended for transfusion purposes:
- A Blood Bank ID armband must be attached to the patient’s wrist at time of blood draw.
- The tube may be either labeled by hand at the bedside with ink on an adhesive label or with a computer-generated label. Either label must contain the following information:
- Complete first and last name. It is acceptable to hand write name if cutoff on computer label.
- Medical record number
- Date and time of collection
- Name or initials of the person collecting the sample.
- Blood Bank ID armband identifiers should be on tube, either in sticker form or hand written.
NOTE: Sample will need to be redrawn if missing any of the above information.
For patients under the age of four months, when sample is intended for transfusion purposes and/or cord blood samples:
- Complete first and last name
- Medical record number
- Date and time of collection
- Name or initials of the person collecting the sample.
Collection Method
Venipuncture
Minimum Volume
Adult | 2 mL |
Pediatric | 2 mL |
Container
Pink Top EDTA
Preferred Specimen
EDTA Whole Blood
Acceptable Specimens
EDTA Whole Blood
Reference or Target Ranges
Not defined for this assay
Critical Value
Not defined for this assay
Reasons for Rejection
Hemolysis Threshold | Gross hemolysis |
Icterus Threshold | N/A |
Lipemia Threshold | N/A |
Interfering Substances/Other | Missing any of the following: Complete first and last name, Medical record number, Date and time of collection, or Name or initials of the person collecting the sample. |
Specimen Stability
Ambient | 72 Hours |
Refrigerated (4°C to 8°C) | 72 Hours |
Frozen(-70°C to -20°C) | N/A |
Performance Information
Days and Time Performed | 24/7 |
Expected Turn Around Time | Routine: within 4 hours of arrival in lab |
Stat Availability | No |
Performing Bench | UH Blood Bank, WCH Blood Bank |
Methodology/Method Description | Manual – Tube Method |
CPT Codes
86902