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Test Code Antigen Typing Antigen Typing

Useful For

Antigen typing is performed on donor units to prevent transfusion of antigen positive red blood cells to patients with clinically significant alloantibodies. Antigen typing may also be performed on patient red blood cells to assist in antibody identification, HDN evaluation, phenotyping of sickle cell patients, phenotyping of patients with warm autoantibodies who have not been transfused within the previous three months, or as part of an organ transplant work-up.

Patient Preparation

No special patient preparation required

Special Collection Instructions

Sample labeling requirements:
For all patients over the age of four months, when sample is intended for transfusion purposes:

  • A Blood Bank ID armband must be attached to the patient’s wrist at time of blood draw.
  •  
  • The tube may be either labeled by hand at the bedside with ink on an adhesive label or with a computer-generated label. Either label must contain the following information:
    • Complete first and last name. It is acceptable to hand write name if cutoff on computer label.
    •  
    • Medical record number
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    • Date and time of collection
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    • Name or initials of the person collecting the sample.
    •  
    • Blood Bank ID armband identifiers should be on tube, either in sticker form or hand written.

  • NOTE: Sample will need to be redrawn if missing any of the above information.


For patients under the age of four months, when sample is intended for transfusion purposes and/or cord blood samples:

  • Complete first and last name
  •  
  • Medical record number
  •  
  • Date and time of collection
  •  
  • Name or initials of the person collecting the sample.

Collection Method

Venipuncture

Minimum Volume

Adult 2 mL
Pediatric 2 mL

Container

Pink Top EDTA

Preferred Specimen

EDTA Whole Blood

Acceptable Specimens

EDTA Whole Blood

Reference or Target Ranges

Not defined for this assay

Critical Value

Not defined for this assay

Reasons for Rejection

Hemolysis Threshold Gross hemolysis
Icterus Threshold N/A
Lipemia Threshold N/A
Interfering Substances/Other Missing any of the following: Complete first and last name, Medical record number, Date and time of collection, or Name or initials of the person collecting the sample.

Specimen Stability

Ambient 72 Hours
Refrigerated (4°C to 8°C) 72 Hours
Frozen(-70°C to -20°C) N/A

Performance Information

Days and Time Performed 24/7
Expected Turn Around Time Routine: within 4 hours of arrival in lab
Stat Availability No
Performing Bench UH Blood Bank, WCH Blood Bank
Methodology/Method Description Manual – Tube Method

CPT Codes

86902