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Test Code BLAST Blastomyces Antibody, Enzyme Immunoassay, Serum

Useful For

Aiding in the diagnosis of blastomycosis

Reporting Name

Blastomyces Ab, EIA, S

Specimen Type

Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.8 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Heat inactivated specimen Reject

Reference Values

Negative

Reference values apply to all ages.

Method Description

The Omega Blastomyces Total Antibody EIA (enzyme immunoassay) uses microwells coated with purified Blastomyces yeast-phase antigen. Patient specimen is diluted in buffer and incubated in the coated microwell. If present, IgG and IgM antibodies bind to the antigen. The microwells are washed to remove unbound serum components. A secondary antibody, rabbit anti-human IgG and IgM antibody conjugated to horseradish peroxidase, is added to the microwell and incubated. The secondary antibody will bind to the antibody-antigen complexes. The microwells are washed to remove unbound conjugate. Substrate solution containing urea peroxide and tetramethylbenzidine is added to the microwells, causing a color change. After a final incubation period, a stop solution is added to the microwells, and the color change is quantified by measuring the optical density (OD). Specimen OD readings are compared to calibrator cutoff OD readings to determine results.(Package insert: Omega Blastomyces Total Antibody EIA. Immuno-Mycologics, Inc; Revision 01/24/2018)

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86612

86612 (if applicable)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BLAST Blastomyces Ab, EIA, S 7816-2

 

Result ID Test Result Name Result LOINC Value
BLAST Blastomyces Ab, EIA, S 7816-2

Method Name

Enzyme Immunoassay (EIA)

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
SBL Blastomyces Ab, Immunodiffusion, S Yes, (SBL) No

Testing Algorithm

If result is equivocal or positive, Blastomyces antibody by immunodiffusion will be performed at an additional charge.

 

For more information see Meningitis/Encephalitis Panel Algorithm.

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.

Secondary ID

35793