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Test Code CBC Auto CBC with Auto Differential

Aliases

HP with Auto Diff

Useful For

A screening tool to confirm a hematologic disorder, to establish or rule out a diagnosis, to detect an unsuspected hematologic disorder, or to monitor effects of therapies. These counts can be used as clinical guides in the diagnosis or monitoring of many diseases.

Collection Method

Venous draw, Capillary Draw

Minimum Volume

Adult: 1 mL, Pediatric 300 uL

Container

Purple (EDTA)

Preferred Specimen

Whole Blood

Acceptable Specimens

EDTA Whole Blood, BD EDTA MAP tube

Reference or Target Ranges

Click here for reference ranges

Critical Value

Parameter Low value High value
ANC (any age) <0.5 X 10 9/L N/A
WBC (0-18 years old) <2 X 109/L >50 X 109/L
WBC (18 and greater years old) <1 X 109/L >80 X 109/L
Hemoglobin (8 weeks and up) <6 g/dL >21 g/dL
Hemoglobin (0-8 weeks) <7 g/dl > 24 g/dl
Hematocrit (any age) <21% >65%
Hematocrit (0-24 hours) <42% >65%
Platelets (any age) <20 X 109/L >1,000 X 109/L

Reasons for Rejection

Hemolysis Threshold N/A
Icterus Threshold N/A
Lipemia Threshold N/A
Other Clotted; QNS, Contaminated; improperly labeled

Specimen Stability

Ambient Up to 24 hrs. at room temp; Slide must be made and stained within 8 hrs.
Refrigerated (4°C to 8°C) 24 Hours
Frozen(-70°C to 0°C) N/A

Performance Information

Days and Time Performed 24/7
Expected Turn Around Time Stat: CBC (w/o differential): Within 1 hour of arrival to lab. No STAT availability for differential.
Routine: Within 4 hours of arrival to lab
Stat Availabilty No
Stat Availabilty CBC (w/o differential): Within 1 hour of arrival to lab.
No STAT availability for differential.
Performing Bench UH, WCH Hematology
Methodology/Method Description Sysmex – Hydrodynamic focusing (DC detection), flow cytometry method(semiconductor laser), SLS hemoglobin method, slides are performed manually if indicated by results.

Special Handling

Room Temp

CPT Codes

85025

Reflex Testing

Smear Reviews, Manual Differentials, Pathology QA reviews, EDTA Plt Phenom