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Test Code ESTF Estrogens, Estrone (E1) and Estradiol (E2), Fractionated, Serum

Reporting Name

Estrogens, E1+E2, fractionated, S

Useful For

Simultaneous high-sensitivity determination of serum estrone and estradiol levels

 

Situations requiring either higher sensitivity estradiol measurement, estrone measurement, or both, including

-As part of the diagnosis and workup of precocious and delayed puberty in females and, to a lesser degree, males

-As part of the diagnosis and workup of suspected disorders of sex steroid metabolism, (eg, aromatase deficiency and 17 alpha-hydroxylase deficiency)

-As an adjunct to clinical assessment, imaging studies, and bone mineral density measurement in the fracture risk assessment of postmenopausal women and, to a lesser degree, older men

-Monitoring low-dose female hormone replacement therapy in postmenopausal women

-Monitoring antiestrogen therapy (eg, aromatase inhibitor therapy)

 

Applications that require moderately sensitive measurement of estradiol including:

-Evaluation of hypogonadism and oligo-amenorrhea in females

-Assessing ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)

 

In conjunction with luteinizing hormone measurements, monitoring of estrogen replacement therapy in hypogonadal premenopausal women

 

Evaluation of feminization, including gynecomastia, in males

 

Diagnosis of estrogen-producing neoplasms in males, and, to a lesser degree, females

Profile Information

Test ID Reporting Name Available Separately Always Performed
E1 Estrone, S Yes Yes
EEST Estradiol, Mass Spectrometry, S Yes Yes

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum Red


Specimen Required


Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube: Red top (Serum gel/SST is not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume:1.2 mL

Collection Instructions: Within 2 hours of collection centrifuge and aliquot serum in plastic vial within 2 hours of collection.

Additional Information: For more information see Steroid Pathways.


Specimen Minimum Volume

0.7mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reference Values

ESTRONE (E1)

CHILDREN*

1 to14 days: Estrone levels in newborns are very elevated at birth but will fall to prepubertal levels within a few days.

Males

Tanner stages#

Mean age

Reference range

Stage I (>14 days and prepubertal)

7.1 years

Undetectable-16 pg/mL

Stage II

11.5 years

Undetectable-22 pg/mL

Stage III

13.6 years

10-25 pg/mL

Stage IV

15.1 years

10-46 pg/mL

Stage V

18 years

10-60 pg/mL

#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (± 2) years. For boys there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.

Females

Tanner stages#

Mean age

Reference range

Stage I (>14 days and prepubertal)

7.1 years

Undetectable-29 pg/mL

Stage II

10.5 years

10-33 pg/mL

Stage III

11.6 years

15-43 pg/mL

Stage IV

12.3 years

16-77 pg/mL

Stage V

14.5 years

17-200 pg/mL

#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (± 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.

*The reference ranges for children are based on the published literature,(1,2) cross-correlation of our assay with assays used to generate the literature data and on our data for young adults.

 

ADULTS

Males: 10-60 pg/mL

Females

Premenopausal: 17-200 pg/mL

Postmenopausal: 7-40 pg/mL

Conversion factor

E1: pg/mL x 3.704=pmol/L (molecular weight=270)

 

ESTRADIOL (E2)

CHILDREN*

1 to14 days: Estradiol levels in newborns are very elevated at birth but will fall to prepubertal levels within a few days.

Males

Tanner stages#

Mean age

Reference range

Stage I (>14 days and prepubertal)

7.1 years

Undetectable-13 pg/mL

Stage II

12.1 years

Undetectable-16 pg/mL

Stage III

13.6 years

Undetectable-26 pg/mL

Stage IV

15.1 years

Undetectable-38 pg/mL

Stage V

18 years

10-40 pg/mL

#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (± 2) years. For boys there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.

 

Females

Tanner stages#

Mean age

Reference range

Stage I (>14 days and prepubertal)

7.1 years

Undetectable-20 pg/mL

Stage II

10.5 years

Undetectable-24 pg/mL

Stage III

11.6 years

Undetectable-60 pg/mL

Stage IV

12.3 years

15-85 pg/mL

Stage V

14.5 years

15-350 pg/mL**

#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (± 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.

*The reference ranges for children are based on the published literature,(1,2) cross-correlation of our assay with assays used to generate the literature data and on our data for young adults.

 

ADULTS

Males: 10-40 pg/mL

Females

Premenopausal: 15-350 pg/mL**

Postmenopausal: <10 pg/mL

**E2 levels vary widely through the menstrual cycle.

Conversion factor

E2: pg/mL x 3.676=pmol/L (molecular weight=272)

 

For International System of Units (SI) conversion for Reference Values, see https://www.mayocliniclabs.com/order-tests/si-unit-conversion.html

Day(s) Performed

Monday through Saturday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82671-Estrogens, fractionated

82670-Estradiol (If applicable)

82679-Estrone (If applicable)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ESTF Estrogens, E1+E2, fractionated, S 53766-2

 

Result ID Test Result Name Result LOINC Value
81418 Estrone, S 2258-2
81816 Estradiol, Mass Spectrometry, S 2243-4

Method Description

The specimen and an internal standard are assayed by liquid chromatography tandem mass spectrometry. The analyte is detected by multiple-reaction monitoring.(Unpublished Mayo method)

Report Available

2 to 6 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Secondary ID

84230

Special Instructions