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Test Code Gent Gentamicin Level

Patient Preparation

No special patient preparation required

Special Collection Instructions

Peak Collect 30 minutes after dose
Trough Collect immediately prior to dosing

Collection Method

Venous collection

Minimum Volume

  • Adults: 1 mL
  • Pediatric: 0.6 mL

Container

Lt Green / Plasma Separator / Lithium Heparin

Preferred Specimen

Light Green Top (lithium heparin) plasma

Acceptable Specimens

Serum:

  • Gold Top SST
  • Red Top

Plasma:

  • Light Geen Top (lithium heparin)
  • Purple or Pink top (K2/K3 EDTA)
  • Dark Green Top (sodium heparin)

 

Reference or Target Ranges

Peak 3 – 12 ug/mL
Trough 0 – 2 ug/mL

Reportable Units

ug/mL

Critical Value

Peak ≥20 ug/mL
Trough ≥2 ug/mL

Reasons for Rejection

 

Hemolysis Threshold 2000 mg/dL
Icterus Threshold 20 mg/dL
Lipemia Threshold 1691 mg/dL
Other QNS, contaminated, improperly Labeled, improper specimen type

Specimen Stability

Ambient 2 hours
Refrigerated (4°C to 8°C) 1 week
Frozen(-40°C to 0°C) 2 weeks

Performance Information

 

Days and Time Performed 24/7
Expected Turn Around Time Stat: 1 hour
Routine: 4 hours
Stat Availability Yes
Performing Bench WH Chemistry
Methodology/Method Description Roche Cobas – Competition assay

Additional Information/Important Notes

Conditional Dosing
Gram positive synergy Adults: within 30 min before 3rd or 4th dose
Pediatrics: at 3rd dose
Pediatric CF extended interval dosing 30 min before 2nd dose
ICN extended interval dosing within 30 min before 4th dose
ICN extended interval dosing (HIE or significant asphyxia) within 20 min before 3rd dose

CPT Codes

80170