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Test Code HIBAG Histoplasma and Blastomyces Antigen, Enzyme Immunoassay, Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Secondary ID

618715

Useful For

Diagnosing Histoplasma capsulatum or Blastomyces dermatitidis infection without differentiation between the organisms

 

Monitor antigen levels following initiation of antifungal treatment

Method Name

Enzyme Immunoassay (EIA)

Reporting Name

Histoplasma/Blastomyces Ag, EIA, S

Specimen Type

Serum

Specimen Minimum Volume

1.2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  72 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat-inactivated specimen Reject

Reference Values

Histoplasma/Blastomyces Antigen Result:

Not Detected

 

Histoplasma/Blastomyces Antigen Value:

Not Detected

Detected: <1.5 ng/mL

Detected: 1.5-25.0 ng/mL

Detected: >25.0 ng/mL

 

Reference values apply to all ages.

Method Description

The assay detects Blastomyces dermatitidis antigen in human serum samples using specific, proprietary antibodies in an enzyme-linked immunosorbent assay format. The detection method involves an enzyme/substrate system with the level of urinary B dermatitidis antigen proportional to the assay signal. The patient specimen result is compared to a cutoff calibrator and a standard curve of a series of assay calibrators (1.25 to 20.00 ng/mL) to determine the presence or absence of antigen, and if present, to establish a quantitative level of B dermatitidis serum antigen.(Package insert: Blastomyces dermatitidis Serum Antigen Detection Kit. Gotham Biotechnology; V2, R2, 01/2023)

Day(s) Performed

Monday through Saturday

Report Available

Same day/1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87449

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HIBAG Histoplasma/Blastomyces Ag, EIA, S 101587-4

 

Result ID Test Result Name Result LOINC Value
619492 Histoplasma/Blastomyces Ag Result 101588-2
619493 Histoplasma/Blastomyces Ag Value 101589-0