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Test Code Hgb Elec Hemoglobin Electrophoresis

Aliases

Hemoglobin Electrophoresis Quantitation with Pathologist Interpretation

Useful For

To differentiate and confirm hemoglobin variants identified using capillary screening methods.

Patient Preparation

No special patient preparation required

Collection Method

Venous collection

Minimum Volume

1 mL

Container

Purple / EDTA

Preferred Specimen

EDTA Whole Blood

Acceptable Specimens

EDTA Whole Blood

Reference or Target Ranges

Hemoglobin A 96 – 98.5
Hemoglobin F 0
Hemoglobin S 0
Hemoglobin A2 1.5 – 4.0
Hemoglobin C 0

Reportable Units

% (percentage)

Critical Value

Not defined for this assay

Reasons for Rejection

Hemolysis Threshold N/A
Icterus Threshold N/A
Lipemia Threshold N/A
Other QNS, contaminated, improperly labled, improper specimen type. Avoid aged, improperly stored blood samples; degradation products (or artefacts) may affect the electrophoretic pattern after 7 days storage.

Specimen Stability

Ambient N/A
Refrigerated (2°C to 8°C) 7 Days
Frozen(<-20°C to 0°C) N/A

Performance Information

Days and Time Performed Monday – Friday 8 AM – 4:40 PM
Expected Turn Around Time Within 3 days of arrival in lab
Stat Availabilty No
Performing Bench Special Chemistry
Methodology/Method Description Sebia Capillarys 2 or Titan Plus – Capillary or citrate agar electrophoresis

Additional Information/Important Notes

Capillary electrophoresis has not been validated in the neonate/newborn population, birth to 28 days of age.
Repeat hemoglobin electrophoresis at 4 month of age may be suggested.
Citrate agar electrophoresis is not orderable, is a reflex test, and will be performed in conjunction with capillary hemoglobin electrophoresis prior to pathologist interpretation as necessary.

CPT Codes

83020

Reflex Testing

If hemoglobin S is present, it is confirmed by Sickle Screen. If hemoglobin C is present, or there is unclear separation of hemoglobin variants, confirmation by citrate agar electrophoresis is performed.