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Test Code LEPDT Leptospira, IgM, Serum

Secondary ID

65183

Useful For

Aiding in the diagnosis of leptospirosis

 

This test is not useful for establishing cure or response to therapy.

Method Name

Enzyme-Linked Immunoassay Dot (Immunodot)

Reporting Name

Leptospira, IgM, S

Specimen Type

Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.3 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.

Additional Information: If acute and convalescent specimens are being obtained to determine seroconversion, they should be collected 2 or more weeks apart.


Specimen Minimum Volume

0.1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

Negative

Method Description

The GenBio IgM ImmunoDOT Leptosirosis test utilizes an enzyme-linked immunoassay dot technique for the detection of IgM antibodies. Leptospira biflexa, serovar Patoc 1 strain antigens are dispensed as discrete dots onto a solid membrane. After adding the test specimen to a reaction cuvette, an assay strip is inserted, allowing patient antibodies reactive with the test antigens to bind to the strip's solid support member. Alkaline phosphatase conjugated goat antihuman IgM antibodies are allowed to react with bound patient antibodies. Finally, the strip is transferred to an enzyme substrate reagent, which reacts with bound alkaline phosphatase to produce an easily seen, distinct spot.(Package insert: ImmunoDOT Leptospira IgM. GenBio; 06/20/2022)

Day(s) Performed

Monday, Thursday

Report Available

Same day/1 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86720

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LEPDT Leptospira, IgM, S 23201-7

 

Result ID Test Result Name Result LOINC Value
65183 Leptospira, IgM, S 23201-7

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.