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Test Code Syphilis Ab Syphilis Antibody, IgG and IgM

Patient Preparation

No special patient preparation required

Collection Method

Venous collection

Minimum Volume

  • Adults: 2 ml
  • Pediatric: 1 mL

Container

Gold Top / Serum Separator / Additive Free

Preferred Specimen

Gold Top SST Serum

Acceptable Specimens

Serum


  • Gold Top SST

  • Red Top

Plasma

  • Light Green Top (Lithium Heparin)

Reference or Target Ranges

Nonreactive

Reportable Units

Reactive/Nonreactive

Critical Value

Not defined for this assay

Reasons for Rejection



<!-- Rejection-->
Hemolysis Threshold
 500 mg/dL
Icterus Threshold
 40 mg/dL
Lipemia Threshold
 2000 mg/dL
Other
 QNS, contaminated, improperly Labeled, improper specimen type

Specimen Stability



<!-- Stability -->
Ambient
 5 days
Refrigerated (4°C to 8°C)
 14 days
Frozen(-40°C to 0°C)
 12 months

Performance Information



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Days and Time Performed
 24/7
Expected Turn Around Time
 Stat: 1 hour
Routine: 4 hours
Stat Availability
 Yes
Performing Bench
 WCH Chemistry
Methodology/Method Description
 Roche Cobas – Two site sandwich assay

CPT Codes

86780

Reflex Testing

All positive results will reflex to RPR rapid testing for titer. If syphilis results are reactive and RPR results are negative, TPPA-Mayo will be sent out for testing. This test cannot be used for congenital syphilis, except indirectly if the mother of a newborn is positive with this and the reflexed RPR on the same sample.