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Test Code T/B Qn Flow T- and B-Cell Quantitation by Flow Cytometry

Useful For

Please see the Flow Cytometry Flow Chart for ordering instructions
Follow-up and diagnostic evaluation of primary immunodeficiency’s, including severe combined immunodeficiency
Immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, and other immunological conditions where such treatment is utilized
Assessment of immune reconstitution post hematopoietic cell transplantation

Patient Preparation

No special patient preparation required

Collection Method

Venous draw

Minimum Volume

2 mL

Container

Purple (EDTA)

Preferred Specimen

Whole Blood

Acceptable Specimens

EDTA Whole Blood

Reference or Target Ranges

Appropriate age-related referance values provedied on report

Reportable Units

cells/μL

Critical Value

Not defined for this assay

Reasons for Rejection

 

 

Hemolysis Threshold N/A
Icterus Threshold N/A
Lipemia Threshold N/A
Other QNS, contaminated, improperly labled, improper specimen type, specimen clotted

Specimen Stability

Ambient 24 hours
Refrigerated (4°C to 8°C) Do not refrigerate
Frozen(-40°C to 0°C) Do not freeze

Performance Information

 


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Days and Time Performed Monday – Friday 9AM – 5PM
 
Expected Turn Around Time 24 hours
Stat Availabilty No
Performing Bench Flow Cytometry
Methodology/Method Description BD FACSCanto II – Flow Cytometry, Dual Platform (CBC w. BD multitest CD3/CD8/CD45/CD4 and BD multitest CD3/CD16+CD56/CD45/CD19)

Additional Information/Important Notes

This will not assess a leukemia/lymphoma or related malignancy. Proper test is Flow LLI (Leukemia Lymphoma immunophenotyping).

CPT Codes

86357;86355;86358;86360