Home
HelpTest Code TDP Thiamine (Vitamin B1), Whole Blood
Useful For
Assessment of thiamine deficiency
Measuring thiamine levels in patients with behavioral changes, eye signs, gait disturbances, delirium, and encephalopathy; or in patients with questionable nutritional status, especially those who appear at risk and who also are being given insulin for hyperglycemia
Secondary ID
42356Shipping Instructions
Ship specimen in amber vial or tube to protect from light.
Specimen Required
Patient Preparation:
1. Patient should fast overnight (12-14 hours); infants should have specimen collected just before next feeding. Water can be taken as needed.
2. For 12 hours before specimen collection, patient should not take vitamin supplements.
Supplies: Amber Frosted Tube, 5 mL (T915)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Amber vial
Specimen Volume: 4 mL
Collection Instructions:
1. Invert 8 to 10 times to mix blood.
2. Transfer whole blood into amber vial or tube and freeze within 24 hours of collection.
Do not spin EDTA tube prior to transferring into amber tube
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Frozen | 28 days | LIGHT PROTECTED |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Thiamin (Vitamin B1), WBSpecimen Type
Whole Blood EDTAReject Due To
| Gross lipemia | Reject |
| Glass vial Clotted specimen |
Reject |
Reference Values
70-180 nmol/L
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
84425
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TDP | Thiamin (Vitamin B1), WB | 32554-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 85753 | Thiamin (Vitamin B1), WB | 32554-8 |
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
Method Description
Samples are extracted with methanol and an isotopically labeled internal standard. Following centrifugation, an aliquot of the supernatant is dried down and reconstituted. The analyte is then detected using liquid chromatography-tandem mass spectrometry.(Unpublished Mayo method)