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Test Code TULAB Francisella tularensis Antibody, IgM and IgG, ELISA, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Secondary ID

605950

Useful For

Aiding in the diagnosis of tularemia caused by Francisella tularensis

 

This test should not be used as a test of cure as it is not quantitative. Patients may remain seropositive for months to years following resolution of disease.

Profile Information

Test ID Reporting Name Available Separately Always Performed
TULM F. tularensis Ab, IgM ELISA, S No Yes
TULG F. tularensis Ab, IgG ELISA, S No Yes
TULI F. tularensis Interpretation No Yes

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

F. tularensis Ab, IgM/IgG ELISA, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  30 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat inactivated specimen Reject

Reference Values

Negative

 

Reference values apply to all ages.

Method Description

The enzyme-linked immunosorbent assay is an immunoassay that is particularly suited to the determination of antibodies in various kinds of samples. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase detects and binds to the immune complex. The colorless substrate p-nitrophenylphosphate is then converted into the colored product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically. (Package insert: Francisella tularensis IgG/IgM ELISA, Immuno-Biological Laboratories Inc; V 142.6)

Day(s) Performed

Tuesday, Thursday

Report Available

Same day/1 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86668 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TULAB F. tularensis Ab, IgM/IgG ELISA, S 93715-1

 

Result ID Test Result Name Result LOINC Value
TULM F. tularensis Ab, IgM ELISA, S 93716-9
TULG F. tularensis Ab, IgG ELISA, S 93717-7
TULI F. tularensis Interpretation 93718-5

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.