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Test Code Tob Tobramycin Level

Patient Preparation

No special patient preparation required

Special Collection Instructions

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Peak Collect 30 minutes after dose
Trough Collect immediately prior to dose

Collection Method

Venous collection

Minimum Volume

  • Adults: 1 mL
  • Pediatric: 0.6 mL

Container

Lt Green / Plasma Separator / Lithium Heparin

Preferred Specimen

Light Green Top (lithium heparin) plasma

Acceptable Specimens

Serum:

  • Gold Top SST

  • Red Top

Plasma:
  • Light Geen Top (lithium heparin)
  • Purple or Pink Top(K2/K3 EDTA)
  • Dark Green Top (sodium heparin)

Reference or Target Ranges

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Peak 3 – 12 ug/mL
Trough <2 ng/mL

Reportable Units

ug/mL

Critical Value

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Peak or random >20 ug/mL
Trough >2 ug/mL

Reasons for Rejection



<!-- Rejection-->
Hemolysis Threshold
 800 mg/dL
Icterus Threshold
 30 mg/dL
Lipemia Threshold
 750 mg/dL
Other
 QNS, contaminated, improperly Labeled, improper specimen type

Specimen Stability



<!-- Stability -->
Ambient
 4 hours
Refrigerated (4°C to 8°C)
 3 days
Frozen(-40°C to 0°C)
 1 month

Performance Information



<!-- Performance information -->
Days and Time Performed
 24/7
Expected Turn Around Time
 Stat: 1 hour
Routine: 4 hours
Stat Availability
 Yes
Performing Bench
 UH Chemistry
Methodology/Method Description
 Roche Cobas – Homogeneous competition principle

Additional Information/Important Notes

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Conditional dosing
Gram positive synergy Adults: within 30 min before 3rd or 4th dose
Pediatrics: at 3rd dose
Pediatric CF extended interval dosing 30 min before 2nd dose
ICN extended interval dosing within 30 min before 4th dose
ICN extended interval dosing (HIE or significant asphyxia) within 30 min before 3rd dose

CPT Codes

80200