Test Code Tob Tobramycin Level
Patient Preparation
No special patient preparation required
Special Collection Instructions
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| Peak | Collect 30 minutes after dose |
| Trough | Collect immediately prior to dose |
Collection Method
Venous collection
Minimum Volume
- Adults: 1 mL
- Pediatric: 0.6 mL
Container
Lt Green / Plasma Separator / Lithium Heparin
Preferred Specimen
Light Green Top (lithium heparin) plasma
Acceptable Specimens
Serum:
- Gold Top SST
- Red Top
Plasma:
- Light Geen Top (lithium heparin)
- Purple or Pink Top(K2/K3 EDTA)
- Dark Green Top (sodium heparin)
Reference or Target Ranges
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| Peak | 5 – 10 ug/mL |
| Trough | 1 -2 ug/mL |
Random 4-10ug/mL
Reportable Units
ug/mL
Critical Value
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| Peak or random | >20 ug/mL |
| Trough | >2 ug/mL |
Reasons for Rejection
<!-- Rejection-->
| Hemolysis Threshold | 500 mg/dL |
| Icterus Threshold | 20 mg/dL |
| Lipemia Threshold | 700 mg/dL |
| Other | QNS, contaminated, improperly Labeled, improper specimen type |
Specimen Stability
<!-- Stability -->
| Ambient | 4 hours |
| Refrigerated (4°C to 8°C) | 3 days |
| Frozen(-40°C to 0°C) | 1 month |
Performance Information
<!-- Performance information -->
| Days and Time Performed | 24/7 |
| Expected Turn Around Time |
Stat: 1 hour Routine: 4 hours |
| Stat Availability | Yes |
| Performing Bench | UH Chemistry |
| Methodology/Method Description | Siemens Atellica - Immunoassay |
Additional Information/Important Notes
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| Conditional dosing | |
|---|---|
| Gram positive synergy | Adults: within 30 min before 3rd or 4th dose Pediatrics: at 3rd dose |
| Pediatric CF extended interval dosing | 30 min before 2nd dose |
| ICN extended interval dosing | within 30 min before 4th dose |
| ICN extended interval dosing (HIE or significant asphyxia) | within 30 min before 3rd dose |
CPT Codes
80200