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Test Code Trig Triglycerides

Patient Preparation

No special patient preparation required

Collection Method

Venous collection

Minimum Volume

  • Adults: 0.8 ml
  • Pediatric: 0.5 ml

Container

Lt Green / Plasma Separator / Lithium Heparin

Preferred Specimen

Light Green Top (lithium heparin) plasma

Acceptable Specimens

Serum:

  • Gold Top SST

  • Red Top

Plasma:
  • Light Geen Top (Lithium heparin)
  • Purple Top(K2 EDTA)

Reference or Target Ranges

<150 mg/dL

Reportable Units

mg/dL

Critical Value

Not defined for this assay

Reasons for Rejection



<!-- Rejection-->
Hemolysis Threshold
 500 mg/dL
Icterus Threshold
 40 mg/dL
Lipemia Threshold
 N/A
Other
 QNS, contaminated, improperly Labeled, improper specimen type

Specimen Stability



<!-- Stability -->
Ambient
 8 hours
Refrigerated (4°C to 8°C) Plasma: 15 days
Serum: 10 days
Frozen(-40°C to 0°C)
 3 months

Performance Information



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Days and Time Performed
 24/7
Expected Turn Around Time
 Stat: 1 hour
Routine: 4 hours
Stat Availability
 Yes
Performing Bench
 UH Chemistry, WCH Chemistry
Methodology/Method Description
 Roche Cobas – Enzymatic colorimetric test using a lipoprotein lipase from microorganisms for the rapid and complete hydrolysis of triglycerides to glycerol followed by oxidation to dihydroxyacetone phosphate and hydrogen peroxide. The hydrogen peroxide produced then reacts with 4-aminophenazone and 4-chlorophenol under catalytic action of peroxidase to form a red dye dyestuff (Trinder endpoint reaction). The color intensity of the red dyestuff formed is directly proportional to the triglyceride concentration and can be measured photometrically.

CPT Codes

84478