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Test Code Type and Crossmatch Type and Crossmatch

Aliases

Type and Cross

Useful For

Discover the ABO and Rh antigens on recipient red cells and presence of anti-ABO antibodies in recipient plasma. Necessary to prevent ABO incompatible blood product transfusions. In addition, plasma is tested against red blood cells from a potential unit selected to transfuse to do a final check for compatibility or incompatibility. Required before and non-emergency transfusion. Type and Screen can be converted to type and cross in 30 minutes.

Patient Preparation

No special patient preparation required

Special Collection Instructions

Sample labeling requirements:
For all patients over the age of four months, when sample is intended for transfusion purposes:

  • A Blood Bank ID armband must be attached to the patient’s wrist at time of blood draw.

  • The tube may be either labeled by hand at the bedside with ink on an adhesive label or with a computer-generated label. Either label must contain the following information:
    • Complete first and last name. It is acceptable to hand write name if cutoff on computer label.

    • Medical record number

    • Date and time of collection

    • Name or initials of the person collecting the sample.

    • Blood Bank ID armband identifiers should be on tube, either in sticker form or hand written.

  • NOTE: Sample will need to be redrawn if missing any of the above information.

For patients under the age of four months, when sample is intended for transfusion purposes and/or cord blood samples:

  • Complete first and last name

  • Medical record number

  • Date and time of collection

  • Name or initials of the person collecting the sample.

Collection Method

Venipuncture

Minimum Volume

Adult 2 mL
Pediatric 1 mL in two MAP tubes

Container

Pink Top EDTA

Preferred Specimen

5 mL EDTA Whole Blood

Acceptable Specimens

EDTA Whole Blood

Reference or Target Ranges

Not defined for this assay

Critical Value

Positive screen with patients currently in OR

Reasons for Rejection

Hemolysis Threshold
Gross hemolysis
Icterus Threshold
N/A
Lipemia Threshold
N/A
Interfering Substances/Other
Missing any of the following: Complete first and last name, Medical record number, Date and time of collection, or Name or initials of the person collecting the sample.

Specimen Stability

Ambient
72 Hours
Refrigerated (4°C to 8°C)
72 Hours
Frozen(-70°C to -20°C)
N/A

Performance Information

Days and Time Performed
24/7
Expected Turn Around Time
Stat: within 1 hour of arrival in lab
Routine: within 4 hours of arrival in lab

IMPORTANT NOTE: Due to the complex nature of some antibodies, testing may take an extended amount of time and may call for additional testing to be sent out for verification of antibodies. Because of this, these turn around times are for reference only.
Stat Availability
Yes
Performing Bench
UH Blood Bank, WCH Blood Bank
Methodology/Method Description

Ortho Vision Analyzer Gel Cards
Manual Gel Cards
Tube Method

Panel Components

  • ABORh
  • Antibody Screen
  • Option to select blood products (RBC, FFP, PLT, Cryo)