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HelpTest Code VIP Vasoactive Intestinal Polypeptide, Plasma
Reporting Name
Vasoactive Intestinal Polypeptide,PUseful For
Detecting vasoactive intestinal polypeptide-producing tumors in patients with chronic diarrheal diseases
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Plasma EDTAOrdering Guidance
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot plasma into a plastic vial. Freeze immediately.
Specimen Minimum Volume
0.55 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 90 days |
Reference Values
<86 pg/mL
Day(s) Performed
Monday, Thursday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
84586
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| VIP | Vasoactive Intestinal Polypeptide,P | 3125-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8150 | Vasoactive Intestinal Polypeptide,P | 3125-2 |
Method Description
Vasoactive intestinal polypeptide (VIP) enzyme-linked immunosorbent assay is a two-step competitive immunoassay. VIP in the patient sample competes with a biotin-labelled antigen for a limited number of anti-VIP antibody binding sites on the microplate wells during the first incubation. After a wash step to remove unbound and excess material the samples are incubated with a streptavidin anti horseradish peroxidase conjugate to form a biotin conjugate complex. After washing, an enzyme substrate is added and incubated. Following the incubation, the reaction is stopped by addition of a stopping solution. Antibody-analyte complex is detected by absorbance measurement at 450 nm wavelength. The absorbance measured is inversely proportional to the concentration of VIP in the samples and calibrators.(Unpublished Mayo method)
Report Available
2 to 4 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.