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Test Code Vit B12 Vitamin B12 Level

Patient Preparation

Fasting recommended

Special Collection Instructions

Samples should not be taken from patients receiving therapy with high biotin doses (i.e. >5 mg/day) until at least 8 hours following the last biotin administration.

Collection Method

Venous collection

Minimum Volume

  • Adult:1 mL
  • Pediatric: 0.6 mL

Container

Lt Green / Plasma Separator / Lithium Heparin

Preferred Specimen

Light Green Top (lithium heparin) plasma

Acceptable Specimens

Serum:

  • Gold Top SST
  • Red Top

Plasma:

  • Light Geen Top (lithium heparin)
  • Purple or Pink Top (K2/K3 EDTA)
  • Dark Green Top (sodium heparin)

 

Reference or Target Ranges

232 – 1245 pg/mL

Reportable Units

pg/mL

Critical Value

Not defined for this assay

Reasons for Rejection

Hemolysis Threshold 400 mg/dL
Icterus Threshold 40 mg/dL
Lipemia Threshold 3000 mL
Other QNS, contaminated, improperly Labeled, improper specimen type

Specimen Stability

 

Ambient Not listed, refrigerate ASAP
Refrigerated (4°C to 8°C) Serum SST: 24 hours
All other tubes: 48 hours
Frozen(-40°C to 0°C) 2 weeks

Performance Information

 

Days and Time Performed 24/7
Expected Turn Around Time Routine: 4 hours
Stat Availability No
Performing Bench UH Chemistry
Methodology/Method Description Roche Cobas – Two site 27 minute immunoenzymatic sandwich assay

CPT Codes

82607