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HelpTest Code Vit D Lvl Vitamin D Level
Aliases
25 OH Vitamin D Level
Patient Preparation
No special patient preparation required
Collection Method
Venous collection
Minimum Volume
- Adult:1 mL
- Pediatric: 0.8 mL
Container
Lt Green / Plasma Separator / Lithium Heparin
Preferred Specimen
Light Green Top (lithium heparin) plasma
Acceptable Specimens
Serum:
- Gold Top SST
- Red Top
Plasma:
- Light Geen Top (lithium heparin)
- Purple or Pink Top (K2/K3 EDTA)
Reference or Target Ranges
30 – 80 ng/mL
Reportable Units
ng/mL
Critical Value
Not defined for this assay
Reasons for Rejection
| Hemolysis Threshold | 600 mg/dL |
| Icterus Threshold | 66 mg/dL |
| Lipemia Threshold | 300 mL |
| Other | QNS, contaminated, improperly Labeled, improper specimen type |
Specimen Stability
| Ambient | 8 hours |
| Refrigerated (4°C to 8°C) | 4 days |
| Frozen(-40°C to 0°C) | 24 weeks |
Performance Information
| Days and Time Performed | 24/7 |
| Expected Turn Around Time | Routine: 4 hours |
| Stat Availability | No |
| Performing Bench | UH Chemistry |
| Methodology/Method Description | Roche Cobas – Competition principle |
Additional Information/Important Notes
| Interpretive Data | |
|---|---|
| Deficiency | <20 ng/mL |
| Insufficiency | 20 – 29 ng/mL |
| Optimum level | 30-80 ng/mL |
| Possible toxicity | >80 ng/mL |
A Vitamin D level of 80 ng/mL is the lowest reported level associated with toxicity in patients without primary hyperthyroidism who have normal renal function. Most patients with toxicity have levels >150 ng/mL. The reference range for total 25-OH Vitamin D is based on the correlation with physiological parameters that include parathyroid hormone concentration and calcium absorption. The range is not based on the distribution of levels in an apparently healthy population. The exact 25-OH Vitamin D level reflecting optimal body stores is currently unknown.
CPT Codes
82306